Evaluation of association between polymorphisms in IL17A, IL17F and IL2 genes and chronic periodontal disease in some health care centers, north Tehran, 2015-2016

Periodontal diseases encompass a wide range of oral diseases in which world health organization concerns about them. The literature already includes several reports of genetics leading role in the development and intensity of periodontal diseases. The purpose of the present study was to investigate the association between IL-17A,F gene polymorphisms and susceptibility to chronic periodontitis in an Iranian population.
Material and Totally 99 patients with chronic periodontal disease and 75 healthy periodontal cases or those with gingivitis were selected through clinical factors (GI, PD, BOP, CAL), as the studypopulation. They were then divided into four groups and 3mL peripheral blood sample were collected. Total DNA was extracted from samples and extracted DNA was stored at -80°C until genotyping and polymorphisms detection using restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR). Ethical issues were all considered. Informed consent was obtained from all volunteers before the study began.
Our data of IL-17 polymorphisms did not show a significant difference amongst study groups (p>0.05).
We did not find statistically significant differences between study groups in Iranian population. Stratification analysis based on the severity of the chronic periodontitis indeed declared no significant difference in relation to genotype distribution and allelic polymorphisms frequencies.