Antifungal Activity In Vitro of Aqeous and Total Flavnoids Extracts of Plant Myrtus communis L. against Two Pathogenically Important Fungi, Saprolegnia and Fusarrium Isolated from Rainbow Trout Eggs

In the present study, the efficiency of the total flavonoids and aqueous extracts of M. communis L. tree leaves, as a recognized medicinal plant of Iran flora were assessed in vitro on the growth of the isolated fungi, Saprolegnia and Fusarrium using the agar disc and well diffusion methods in flat-bottom microplates in presence of the various extract concentrations. The isolated fungi were sampled from the fertilized eggs of rainbow trout fish incubation farms. The collected leaves from the natural habitats of Chaharmahal-o-Bakhtiary province at early-summer were extracted by maceration methods with water solvent as well by flavonoids extraction methods with methanol solvent. During the succeeding trials, the antifungal effects of the flavonoids extracts (by the well diffusion method) and the aqueous extract (by the well diffusion method) against isolated Saprolegnia were revealed by MFC (Minimum Fatal Concentration) values 50 and 100 mg/ml, respectively. The only effect of the methanolic and aqueous extracts of M. communis leaves revealed in vitro inhibiting effect on growth of isolated Fusarium by MIC values 25 and 12.5 mg/ml, in disc diffusion and well diffusion method, respectively. The antifungal obtained effects by the extracts had more effective aspects on isolated Saprolegnia in comparison to Fusarium. The results of the study indicate that M. communis could be considered as a potential candidate for designing effective antifungal suitable for treatment of the fish eggs fungal infections.