Proteome Analysis of Canola Root Inoculated with Pseudomonas fluorescens FY32 under Salinity Stress

Canola which is being used for its oil is affected by salinity stress. Salinity is one of the most important abiotic stressful conditions which affects productivity and quality of agricultural products. Pseudomonas fluorescens is a Plant growth promoting rhizobacteria having symbiotic relation with plants that reduces adverse effects and increases plant growth. In this study salinity stress has been applied in two levels (0 and 300 mM NaCl). Absence and presence of P. fluorescens was the other treatment applied to plant root with three replications. Statistical analysis was carried out as split plot based on completely randomized design. In order to evaluate proteome analysis of canola root under salinity stress inoculated with P. fluorescens 2D-PAGE approach was carried out. To reach this goal proteome extract of root was extracted via TCA-acetone method. Isoelectric focusing and SDS-PAGE for 1st and 2nd dimensions, were used, respectively. Acquired gels were analyzed using PD-quest software after staining with silver nitrate. Results demonstrated 216 spots on the gels. Sixteen spots showed significant change as result of salinity stress. Thirteen spots showed change in quantity as result of presence of P. fluorescens FY32. After applying salinity and inoculation PGPR, 23 spots presented changes significantly. After identification of proteins using molecular weight and PI, it has been recognized that most of the responding proteins to salinity and PGPR presence conditions are categorized in metabolic/ energy related pathways. Then, signaling, maintenance/ defense, channel proteins and protein structure/ location related proteins were the ones that had high frequency respectively.