Nano emulsion formulation from essential oil of salvia hypoleuca and investigation of its anti microbial and physicochemical properties

Second metabolites isolated from salvia spices mostly are terpenoids and flavonoids which are bioactive compounds with high healthy effects. Extractions of these materials by solvent causes chemically unstable and lower healthy effects. Encapsulation of essential oils in a lipid carrier such as nano-emulsion is scientific way to reduce this problem. The aim of this research is encapsulation of essential oil of salvia hypoleuca in nano emulsion and investigation of antimicrobial and physicochemical activities of this essential oil. Nano emulsions containing salvia hypoleuca essential oil with tween 80 and span 80 as surfactants with different HLB were produced by using high intensity ultrasound. Determination of size, distribution of particle size and physical stability of nano emulsion over time was investigated by using dynamic light scattering (DLS). Also evaluation the antimicrobial properties against gram-positive bacteria (bacillus cereus and enterococcus faecalis) and gram-negative bacteria (moraxella catarrhalis and klebsiella pneumonia) microdilution method was used. The results showed that the size and distribution of particle size were in the range of 89-141 and 0/36-0/41 and nano emulsions were stable during 60 days. Anti microbial activities of nano emulsion against all bacteria were better than free essential oils.