Synergistic effect of lysozyme on chitosan-based films including Cumin essential oil on the antimicrobial activity

Lysozyme- chitosan-cumin oil films were develop for enhancing the antimicrobial properties of chitosan films. A different concentration lysozyme solution was incorporated in to chitosan 2% film forming solution and 1% oil cumin .The film used for microbial properties of chicken fillets stored at refrigerator. Lysozme and cumin oil releases from the matrix of chitosan film and effect on antibacterial properties such as total mesophilic bacteria, microbial Total bacterial count, Psychrophiles, Coliforms and Pseudomonas . Results showed that 10% lysozyme and 1% cumin oil enhance the efficiency of chitosan film against bacteria in chicken fillet. Statistical results showed that 3 log cfu/g reduced in mesophilic bacteria in 12 days and 3 log cfu/g , 1.1 log cfu/g , 3 log cfu/g in Psychrophiles, Coliforms and Pseudomonas bacteria respectively .So the results showed the synergistic effect of oil cumin and lysozyme in to chitosan –based films . Thus brooding their application enhancing food quality and saftely .