Validation of a method for determining Aflatoxin M1 in yoghurt

Validating for detecting aflatoxin M1 in yoghurt according to Commission Decision BS EN ISO 14501:2007. In this study yoghurt samples prepared with milk powder without aflatoxin (Made in Holland) and then artificially contaminated by adding aflatoxin M1. Analytical method using immunoaffinity column for extraction and a high-performance liquid chromatography (HPLC) with FLD detector for quantification was performed according to the device settings. To achieve the best possible condition to identify and ensure the separation of aflatoxin M1, deploying some important device control such as, control the flow of the mobile phase and the mobile phase constituents was applied. The procedure for determining selectivity, recovery, precision, decision limit (CCα) and detection capability (CCβ) of the method has been reported. The method includes a preliminary clean-up and the average recoveries determined on three different days at the concentration levels of 0.025, 0.050 and 0.075 µg kg⁻¹ were in 74.28%, 80.89 and 86.77% respectively with RSD in the range of 2.56%-8.41%. The interday and interlevel mean recovery value, which has been used to correct routine analysis results, was 80%. The method is rapid, easily automatable with low retention time and high accuracy therefore useful for accurate and precise screening of aflatoxin M1 in yoghurt.