Determination and comparison of the expression levels of various germ cell-specific genes in human bone marrow- and peripheral blood-derived mesenchymal stem cells

A number of reports have shown that mesenchymal stem cells (MSCs) from various sources, expressed a number of germ cell (GC)-specific genes innately but there wasn’t any complete and quantitative study. So that, in this study we determined and compared the expression levels of some famous GC-specific genes in human male bone marrow (BM)- and peripheral blood (PB)-MSCs.
Mehtods: Human BM- and PB-MSCs were isolated using a density gradient factor and centrifuging. Then, Passage 3 of both cell types were characterized by flow cytometric analysis and differentiation test and then evaluated by real time RT-PCR for the expression levels of GC-specific genes.

Both of the cell types obviously expressed c-kit, Dazl, Fragilis, Itgb1, Nanos3, Oct4 and Blimp1 at high levels and except Itgb1 which was expressed at a higher level in PB-MSCs than in BM-MSCs (p

Overall, it could be said that BM- and PB-MSCs have a little bit different GC-specific gene expression pattern. Although, these data are very important, so many detailed and creative research studies still needed in order to find the way of using MSCs in cell-based therapies of infertility.