THE EFFECT OF GARLIC EXTRACT S ON EXPRESSION OF TNF- Α AND IFNΓ BY PERIPHERAL BLOOD MONONUCLEAR CELLS OF PATIENTS WITH DIABETES MELLITUS TYPE II ADMITTED TO GANJAVIAN DEZFUL HOSPITAL IN 2011-2013

Usually excess feeding in stored in the body as fat and leads to overweight. One of the causes of catching type 2 diabetes is obesity. Proinflammatory cytokines such as TNF- α and IFNγ can damage insulin signaling in insulin-sensitive tissues. In this study, the effect of garlic extract on the expression of TNF-α and IFNγ secreted by the mononuclear cells was measured.
After taking peripheral blood from patient with type 2 diabetes, mononuclear cells were extracted by Ficoll Method. Cells were a culture with two different concentration of garlic extract (10% and 30%) for 48 and 24 hours. Then for measuring TNF-α and IFNγ release level, ELISA method was used.
Garlic extract can reduce the amount of TNF-α secretion from cells mononuclear, although the timing and concentration of the extract had no effect on the results. Moreover, garlic extract had no effect on the secretion of IFNγ.
Using garlic extract can be adopted therapeutic strategies aimed at adipose tissue inflammation and insulin resistance that is useful for the prevention and treatment of type 2 diabetes.