Molecular Assay of Staphylococcal Enterotoxin E in Synovial Fluid of Patients with Rheumatoid Arthritis

The role of Staphylococcal enterotoxin E (Superantigen E) in rheumatoid arthritis pathogenesis has been considered. This paper aimed at determining Staphylococcal enterotoxin E in the synovial fluid of rheumatoid arthritis patients.
In this study, 100 synovial fluid samples of patients with rheumatoid arthritis were examined. The primers pairs were designed based on the S. baureus enterotoxin type E (entE) gene, GenBank: M21319.1. All samples were subjected to DNA extraction separately. Then, polymerase chain reaction (PCR) was implemented. Data were analyzed by descriptive statistics.
The PCR results indicated that Staphylococcal enterotoxin E gene existed in synovial fluid samples of 25% of patients with rheumatoid arthritis, with a high percentage.
The study results revealed that a high percentage of patients with rheumatoid arthritis have Staphylococcal enterotoxin type E gene in their synovial fluid. However, further studies are needed to assess other Staphylococcal enterotoxins. This finding may provide a model for diagnosing rheumatoid arthritis disease. The results of this study have presented some evidence regarding endogenous origin of involved superantigen in patients with rheumatoid arthritis.