Effects of Testosterone on Analgesia in Formalin-Treated Mice

So far, various reports have been presented on the relationship between sex hormones and gender-related differences in pain and analgesia in humans and laboratory animals. The purpose of this study was to investigate the effect of testosterone hormone and spironolactone anti-androgen drug on morphine-induced analgesia in male mice using formalin test.
In this study, 80 male mice were divided into 10 groups (N=8); normal saline (control), sesame seed oil (as testosterone solvent), testosterone (5 and 10 mg/kg body weight), spironolactone, morphine, sesame seed oil morphine, testosterone (5 and 10 mg/ kg body weight) morphine and spironolactone morphine. Formalin test was performed in all the mice, and data were analyzed by one-way ANOVA.
The results showed that sesame seed oil morphine (p It can be concluded that testosterone has analgesic effects on the chronic phase of the pain. On the other hand, spironolactone may have hyperalgesic effects due to its anti-androgenic properties.