Detection of TEM beta-lactamase Gene in Escherichia Coli Isolated from Urinary Tract Infection of referred to the clinic boarding Robat Karim to the molecular method

Aim and Urinary tract infections is one of the most common infectious diseases whichmany factors are involved, but bacteria such as E.coli is the most important agent of urinary tract infections. Antibiotic resistance as a major problem in the treatment and control of these infections is considered. The aim of this study was to determine the genes that cause resistance to betalactam family of antibiotics on E.coli isolated from urinary tract infections in Robat karim city.
Material & In the present Cross-sectional study which was conducted over a period of 6 months, 123 samples of E.coli were collected from Robat karim hospitals for molecular analysis of TEM genes, causing antibiotic resistance by (PCR) method.Data were analyzed using SPSS statistical test.
PCR showed that the gene frequency of TEM (80%) and the highest and lowest prevalent of genes were related toTEM in E.coli isolated from urinary tract infections respectively.
According to the high prevalence of resistance to beta-lactam antibiotics, the current study showed that the noted geneTEM play an important role in facilitating the spread of antimicrobial resistance in this region