Simultaneous Determination of Hydrochlorothiazide and Folic Acid in Pharmaceutical and Biological Samples using a New Modified Nanostructure Sensor

A sensor based on carbon paste electrode (CPE) modified with benzoylferrocene (BF) and NiO nanoparticls (NiO/NPs) was used for the highly sensitive voltammetric and electrocatalytic measurement of hydrochlorothiazide (HCT) and folic acid (FA). The NiO/NPs were synthesized via a direct chemical precipitation method and were characterized with X-ray diffraction (XRD), and scanning electron microscopy (SEM) techniques. The obtained sensor represented a suitable and powerful electron mediating behavior along with excellent-separated oxidation peaks of HCT and FA. The acquired peak currents from square wave voltammetry (SWV) technique were linearly dependent on HCT and FA concentrations in the ranges of 1.0–500.0 and 50.0–500.0 µmolL-1 with limits of detection equal to 0.14 and 4.3 µmolL-1, respectively. This mediator/nanoparticle modified electrode was applied for the detection and measurement of HCT and FA in pharmaceutical and biological samples.