Biosynthesis of Silver Nanoparticles Using Fusarium oxysporum Isolated from Pea (Cicer arietinum)

In recent years there has been an increased interest to use microorganisms as bionanofactories in nanoparticles synthesis. Secreting high amount of proteins, fungi have advantages over other microorganisms. In the present study 15 Fusarium oxysporum isolates from pea were used for the extracellular biosynthesis of silver nanoparticles. After the addition of AgNo3 solution (final concentration 10-3M), the crude cell filtrate of F. oxysporum changed from colorless to brown color indicating the formation of silver nanoparticles (AgNPs). Aliquots of the cell filtrate were removed and subjected to UV-vis spectroscopy at different time intervals to confirm the formation and stability of AgNPs. Nanoparticles showed an absorption peak at 420nm in UV-visible spectrum corresponding to the Plasmon resonance of AgNPs. Further analysis was carried out by X-ray diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR). Broadening the XRD peaks denote the small size of AgNPs. FTIR analysis confirmed the stability of AgNPs by biomolecules capping agents even 5 months after their production.