The Study of Anticancer Effect of Magnetic Chitosan-Hydroxyurea Nanodrug on HeLa Cell line

Drug nanocarriers have usually optimized features which facilitate cellular uptake and increase drug efficacy, prevent drug degradation against enzymatic factors and decrease drug complications by targeted drug delivery to cancer cell. This study aimed to determine the anti-cancer nature of the synthesized magnetic Chitosan-hydroxyurea nanodrug on HeLa cell line, cervical cancer, and to determine the effective dose of the nanoparticle in order to remove cancerous cells. In this laboratory study, after analyzing the structure of synthesized magnetic nanoparticles and culture of HeLa cell line, cells were incubated with different concentrations of nanodrug for 48 h. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) method was used in order to evaluate the vital activity of the cells. For analyzing induction of apoptosis, the Annexin-PI kit was used by flow-cytometry. Data were analyzed using one-way ANOVA and independent t-test. The viability of the cells decreased in a dose dependent manner by increasing the concentration of nano-drug hydroxyurea. So, at the concentrations of 1000 (p<0.001) and 2000 (p<0.05) μg/ml, a significant difference was observed compared to the control group. The results also demonstrated that the nanodrug significantly increased apoptosis induction 2.48 times in the treated HeLa cells in comparison with the control group (p=0.0003). Hydroxyurea nanodrug has a cytotoxic enhancement effect on the Hela cancer cell line
and can induce apoptosis