Survey on biochemical identification and hostage spectrum of produced antibacterial compounds by native lactic acid bacteria

Bacteriocins are organic compounds or peptides synthesis via bacterial that kill or inhibit growth of microorganisms and identical species by attacking a specific target. To take into account of bacterial resistance to antibiotics and more demand to food immunity, identification of strains producing bacteriocin with wide spectrum of hostage is necessary. So in this research determined antibacterial identification of five native lactic acid and entrococcus strains by using enzyme treatments such as catalase, lipase, α-amylase, trypsin and α-kimyo-trypsin. Then hostage spectrum of produced bacteriocin by native strains identified by disk-diffusion test. Variance analysis of enzymatic treatment data on supernatant of E6, DL3, H20, N, M, T2, AE2, and L27 showed existence of significant difference at alpha=0.01 for diameter of growth inhibitory zone among supernatant with adjusted pH and other enzymatic treatments. Mean comparison among enzymatic treatment data on supernatant of 15E and EL1 strains showed between diameter of growth inhibitory zone of supernatant with adjusted pH and treated with Lipase, trypsin and alpha-chymotrypsin existed significant difference. Each of native lactic acid bacteria showed inhibitory growth pattern against positive and negative gram bacteria. Inhibitory zone of bacteria supernatant with pH=6 after treating with catalase, lipase and α-amylase was active with respect to antibacterial. This finding suggested that bacteriocin of this native strain has nature of peptide.