Determination of optimum concentration of pituitary gland extract and LHRHa2 hormones in 3-stages injection based on reproduction indices of Arabibarbus grypus

The aim of the project is to assay the effectiveness of different values ofLHRH-a2 combined with carp pituitary extract in 3-stages way on reproduction indices of Arabibarbus grypus that belong to Cyprinidae family and is a native fish in Khuzestan province. Fifty individuals of fish in 4 treatments were studied. Control treatment were injected by 4mg/kg pituitary extracts, first treated: 4mg/kg CPE+ 3μg/kg LHRHa2, second treatment: 4mg/kg CPE+7μg/kg LHRHa2 and third treatment: 4mg/kg CPE+ 10μg/kg LHRHa2, that 40 fish were treated by 3-stages and 10 fish by usual way, were studied and the reproduction indices such as obtained egg and alive baby were measured. The results obtained that the weight of obtained egg (692 ± 111.2), relative fecundity (41.9 ± 3.22), fertilization (94.4 ± 0.89), hatching rate (98.2 ± 0.84), larvae number (10912.8 ± 1817.93), alive percent of baby (70.5 ± 2.3) was increased in second treatment (3-stages way) compared to the other treatments (usual way) (p <0.05). Therefore, 3-stages way in the second treatment dose (4mg/kg CPE+7μg/kg LHRHa2) is the best used way and concentration for artificial reproduction of Shabout brood stock.