EXPERIMENTAL STUDY ON HISTOLOGY OF CIRCUMSTANCE OF WOUND HEALING PROCESS IN COMMON CARP (CYPRINLLS CARPIO)

The present experimental research was carried out to study the histology the inflammatory response and circumstance of wound healing process in the inflicted skin and underlying muscle of common carp.
A number of 30 fishes with average length of 22cm in a recirculating glass aquaria system, at an average temperature at 27°C, were used for this study.
A surgical wound (1.5-2.0cm long x 0.5cm deep) without complication of secondary infections, was made by sterilized scalpel blade into the left dorsal muscle of each anaesthetized fish. At least one wounded fish was sacrificed and sampled at 30 minutes, I, 2, 4, 6, 8, 10, 12, 24 hours and thereafter at 2, 4,6, 8, 16, 24, 32, 40, 50 and 60 days after wounding. The samples were fixed in 10% buffered formalin and 5µ sections were prepared using standard histological method. The sections were stained by the standard H&E staining method. Also periodic acid schiff (PAS) with tartrazin were used for specific tissue features. The stained sections were examined using light microscope.
Re-epithelialization of wounded epidetmis was completed within 4 hours after wounding. Macrophage infiltration began at 2 hours, and myophagia at 6 hours. Fibroplasia and muscle regeneration were initiated at 2 days after wounding. After 16 days, epidermis was normal and dermis was completely linked. The wounded area restored its main components by 16 to 24 days after wounding. This study revealed that a healthy carp is able to have a rapid and effective inflammatory response and also high potential of repairing against surgical wound.