Investigating the effect of simultaneous application of red LED and sodium dikegulac on shoot regeneration and longitudinal growth of olive (Olea europaea L.) cv. Zard explants

فصل 1: Micro-propagation of olive has a long history but strong apical dominance at the shoot regeneration stage always affects its proliferation such that even with cytokinin treatments it is not controllable. This problem limits olive micropropagation. In the present study, we attempted to determine suitable concentrations of sodium dikegulac under red light in order to increase olive shoot regeneration rate. Factorial design was used with a complete randomized design was used with 4 replications and 4 explants per replication. Moreover, we tried to achieve this purpose at the least possible time. The maximum number of regenerated shoots (3.10 ± 0.22 per bud) was observed at 5 mg/L of sodium dikegulac under the control condition (Fluorescent lamps) during 21 days post sub culture. Based on the number of generated shoots, red light had a negative effect when accompanied by sodium dikegulac. Longitudinal growth revealed a downtrend pattern by increasing concentrations of sodium dikegulac. However, the longitudinal growth of explants was significantly higher under the red light compared to the control. Based on results, it seems that sodium dikegulac and red light have antagonistic effects on each other and sodium dikegulac is only efficient on proliferation when it is used under the white light.