Effect of sucrose and ultrasound pretreatment on morphological characteristics and total phenol of Chelcheragh lily in in-vitro condition

To evaluate the effects of sucrose and and ultrasound on Chelcheragh lily (Lilium ledebourii Boiss.) under in-vitro condition, an experiment was conducted in a factorial experiment based on completely randomized design with four replications in tissue culture laboratory of Department of Horticultural Science in Mohageg Ardebili University in 2014. Treatments in this study were sucrose in 3 levels (30, 45 and 60 g L-1) and ultrasound with frequency of 35 kHz in 5 levels (0, 5, 10, 20 and 30 seconds) on the 2 types of explants (bulblet and scale). In bulb explants, the results showed that the highest fresh weight, bulbs and roots weight, root and plantlet length was obtained from 60 g L-1 sucrose + 10 seconds ultrasound, but the least amount of total phenolic content was observed from this treatment combination. The highest total phenolic content was obtained from 60 g L-1 sucrose + 30 seconds ultrasound. All levels of ultrasound had positive effect on number of scales, number of leafs and leaf length, but reduced the amount of chlorophyll. The highest number of roots and number of rooted bulbs was observed in MS medium containing 30 g L-1 sucrose. In both explants, MS medium containing 45 g L-1 sucrose was effected in increase of length of root. In second experiment, ultrasound destroyed the scale explants.