Investigating Growth and Budding of Cuttings and Image Analysis in Some Rose Cultivars Affected by Temperature Factor

Two experiments were conducted to evaluate budding strength of cuttings and imaginary differences of rose leaves as a result of different temperature treatments. In the first trial four different temperatures (1, 3, 5.5 and 80c) along with 3 different layers of stems from which cuttings had been taken were used in a factorial randomized complete block design. The experiment was composed of 12 treatments, 4 replications each one containing 3 samples. Budding features of cuttings which were measured and recorded were: days to budding, sprout diameter and length, number of leaves and sprout fresh weight. Results of this experiment indicated that both temperature and stem layer affected days to budding, sprout length and diameter significantly with middle layer showing maximum sprout length and diameter when compared with the two other layers. To reach the budding stage the top layer was the fastest. Second experiment was designed using two factors in which 5 levels of temperature (-11, 0, 5, 10, 250c) were used to treat leaf samples taken from 4 different cultivars and species of roses (Rosa hybrida 'Blue moon', R. damascene, R. 'Tuscany superb', R. 'Fieta') based on factorial randomized complete block design. According to the results it was determined that green and red color parameters of leaf images could be used as a scale to evaluate the effect of temperature variations on rose leaf. Comparing with blue color, these two colors showed high potential in quick and earlier detection of stresses imposed by environmental temperature variations.