A Survey on hematological parameters of Capoeta Capoeta gracilic in Talar River Qaemshahr, Mazandaran

Capoeta Capoeta gracilic is one of the native and valuable species in south of basin of from the Caspian Sea. The hematological indexes in fish physiology are influential, so the accurate understanding of blood parameters of this fish can be useful to protect of these valuable stocks. In this research 50 pieces Capoeta capoeta gracilic were collected by Electro shocker from two sampling stations in intermediate area of Talar River. The sampling operation was carried out during the reproduction season and the fish were in four age groups 1, 2, 3 and 4, after blood obtaining in the way on caudal peduncle incision in smaller fish and heart ponxion in larger fish and sex determination and age determination, the amount of some of hematologic parameters Including the total count and subtraction of WBC, the total count of RBC, Hb, Hct, and globule indexes (MCH, MCHC, MCV) were measured manually and with conventional laboratory methods and mean and standard error each of the foresaid parameters in different ages and by gender were determined. The amount of blood parameters regardless age and gender is as follows: The total count of WBC (16359.6 ± 656.3) mm3, the count of RBC (1.581 ×106±0.08) mm3, Hb (4.41± 1.15) g/dl, Hct (38.01 ± 3.16) )%(, MCV (187.2 ± 11.99) (fl), MCH
(28.09 ± 7.01) ( pg), MCHC (15.79 ± 4.64) )%( and in differential count of WBC, percent of neutrophils, lymphocytes, monocytes, eosinophile were 17.05 ± 2.14 , 78.54 ± 1.88, 3.43 ± 0.47,1.2 ± 0.13 )%(. The result of statistical analysis did not show signification differences between two sexes (P> 0.05). In studying of the effect of the age also showed the hematological factors in different ages do not have significant differences (P> 0.05). According to the result assessment and diagnosis physiological status of blood indexes and determination them for estimating, saving and keeping of the Capoeta capoeta gracilis stocks is necessary.