Evaluation of the relationship between different polymorphisms of vitamin D receptor gene and renal diseases in patients with type 2 diabetes in Khuzestan province

The purpose of this study was to investigate the association between different polymorphisms of the vitamin D receptor coding gene (Taq1, Bsm1, Apa1, Fok1) with renal disease in patients with type 2 diabetes.

In this case-control study, blood samples were taken from 50 healthy controls and 50 type 2 diabetic patients as control and 50 type 2 diabetic patients with nephropathy. Then, SSP-PCR technique was used to determine the different genotypes of vitamin D receptor polymorphisms and the results were analyzed by SPSS 22 software.

In type 2 diabetic patients with Bb (64%) genotypes were the most frequent genotypes for Bsm1 polymorphism, FF (54%) for Fok1, Aa (84%) for Apa1 and TT (36%) for Taq1. In addition, the probability of Nephropathy with bb genotype (P-Value = 0.007, OR = 6.7730) for Bsm1 polymorphism, ff genotype (OR = infinity and P-Value = 0.01) for Fok1 polymorphism, Aa genotype (OR) = 5.25 and P-Value <0.001) for Apa1 polymorphism and tt genotype (OR = 5.41, P-Value = 0.003) for Taq1 polymorphism increased.

Genotypic frequency of Bsm1, Apa1, Fok1 and Taq1 polymorphisms is related to the incidence of renal disease (Nephropathy) in type 2 diabetic patients. However, the correlation between different polymorphisms and the incidence of kidney disease shows that Apa1 polymorphism has the greatest effect on renal complications in patients with type 2 diabetes. In addition, Bsm1 polymorphism has the highest effect on the incidence of type 2 diabetes.