Improving Rooster Sperm Quality by Adding Different Levels of Naringenin after Freezing-Thawing Process

Artificial insemination is a reproductive technology that uses the best male breeders. Successful artificial insemination is affected by several factors, one of the most important of which is the availability of fertile sperm after the freezing-thawing process. The purpose of this study was to investigate the effect of different concentrations of naringenin after the freezing process. Sperm collection was performed twice a week using abdominal masage. After adding the diluent to the semen samples, the samples were placed in the refrigerator at a temperature of 4°C for equilibration. Samples were then transferred to freezing straws and exposed to nitrogen vapor, then stored in a tank containing liquid nitrogen. After thawing, several parameters containing motility, viability, abnormality, membrane integrity, MDA production, SOD and GPX enzymes and total antioxidant capacity were evaluated. The results indicate that the total and progressive cavity in 100 μM naringenin treatment significantly increased compared to control treatment. The percentage of sperm with morphological abnormalities in different concentrations of naringenin did not differ significantly (p<0.05). The viability and membrane integrity of the sperm cells at 100 μm concentration increased significantly compared to the control group. The results of this experiment indicate that 100 μm naringenin treatment increased glutathione peroxidase and total antioxidant capacity and reduced MDA levels compared to control group. Based on the results of this study, naringenin at 100 μm in a diluent medium improves rooster sperm quality after freeze-thawing.