Effect of Ethylene Diamine Tetra Acetic Acids on Morphological Characteristics and Phytoremediation Capacity of Indian mustard (brassica juncea L) in Nickel Contaminated Soil

Contaminated soil with heavy metal is one of the most important environmental issues in the world. The refining plant is one of the methods of refinement and prevent potential pollution hazards of heavy metals in soil. The purpose of this study, effects of various concentrations of EDTA on some morphological characteristics and the efficiency of EDTA for Increased Ni from soil and to compare their effects for enhancing of Ni extraction with Indian mustard (brassica juncea L). For this purpose, a greenhouse experiment was conducted in completely randomized design with four replications. Soil samples were mixed with 600 mg/kg of nickel sulfate and the seeds were planted in soil of pot. The experimental factor was including 0, 1, 5, 10 and 12 mmol EDTA that after the seed cultivation have been added to the soil. The results of the research showed that The application of different EDTA concentrations decreased morphological characteristics and increased soluble nickel concentrations in soil and transfer it to the inside of the plant and this changes was significant compared to the control treatment. The increase in soluble nickel concentrations in soil and transfer it to the inside of the plant inhibits the absorption of essential elements to the plants shoot and thus, repelled the growing process. In general, Indian mustard (brassica juncea L) due to high nickel resistance can be introduced as a Ni hyper accumulator to EDTA induced phytoextraction technology.  But according to the preventive effects of these substances on the growth of the plant should be used for low concentrations.