Investigation The Antimicrobial Eeffects of Manganese and Iron Doped Zinc Oxide Nanoparticles against Shigella flexneri

In this study, the synthesis of nanoparticles of manganese-zinc oxide and zinc iron oxide by chemical co- precipitation method performed and investigation of structural and morphological properties of related nanoparticles using Fourier Infrared spectrum, X-ray diffraction and Transmission electron microscopy were carried out. Antimicrobial effects against shigella flexneri (PTCC 1234) determined by inhibitory diameter zone method. Synergistic effect of nanoparticle with hydrogen peroxide and hypochlorite sodium also assessed by calculation of the fractional inhibitory concentration (FIC) index. Structural analysis of Mn- ZnO and Fe- ZnO nanoparticle revealed that co- precipitation is a suitable method for synthesis of these nanoparticles. Results of antimicrobial tests showed that Mn- ZnO nanoparticles has more antibacterial effect against shigella flexneri than Fe- ZnO nanoparticles. Inhibitory zone diameter of Mn- ZnO and Fe- ZnO were 25 and 14 mm, respectively. In addition, both doped nanoparticle exhibited more antimicrobial effect than zinc oxide nanoparticle so, doping cause increasing antimicrobial effect of zinc oxide nanoparticle. Furthermore, combination of nanoparticles with hydrogen peroxide and hypochlorite sodium showed synergistic effects which was complete synergistic at high concentration and partial synergistic at low concentrations.