Feeding Artemia larvae with yeast heat shock proteins 82 (HSPs82) to enhance the resistance against abiotic stresses (hyperosmotic and high temperatures)

Feeding farmed Artemia with yeast heat shock proteins is a novel way to protect them from stress conditions during the culture.  In this study, the effect of feeding with stressed new identified Saccharomyces cerevisiae strain YG3-1 yeasts (containing induced heat shock proteins) on the survival of Artemia in stress conditions, was evaluated. For this purpose, heat shock proteins 82 (Hsps 82) of mentioned yeasts were induced by applying the high thermal (30, 35 and 40 ˚C) and high salinity (60, 120, 180 and 240 g.l-1) stresses. After that, two different species of Artemia (Artemia urmiana and A. franciscana) were fed with treated yeasts during the culture. Then, to investigate the effects of S. cerevisiae strain YG3-1 Hsps82 on Artemia survival, after the end of feeding, adult individuals of both species were exposed to authorized high salinity (230 g L-1  and  280 g L-1) and authorized high temperature (35 ˚C  and  37 ˚C) as permitted stress for 48 h (hours). Finally, this administration resulted in the resistance of both species against the high salinity and high temperature (p<0.05). This result was confirmed by analysing total protein of Artemia using SDS-PAGE, and suggests that this administration can be used for enhancing the survival of Artemia in stress conditions.