Study of genetic diversity of some genotypes of gourd using the location of ribosomal and chloroplasty genes

The method of barcoding DNA has a great potential for the revival of archeology and traditional biodiversity studies; therefore, this method has so far been widely used in studies on plants and for purposes of classification and archeology. In this research, 96 plants were planted in greenhouse according to a completely randomized design, and in 4 leaf stage, young leaves were harvested as samples. In the next step, DNA extraction, quantitative and qualitative evaluation, PCR and PCR sequencing were performed and data analysis was performed using Clastal w2 and BioEdit software. The results showed that the nucleotide sequences of the ITS gene and rbcl in the pumpkin family, Guanine were highest (26.9 and 22.6, respectively), adenine was the least frequent (20.3 and 27.4). Estimation of the nucleotide replacement of RBCL and ITS gene in the pumpkin family had high levels of pyrimidine replacement, resulting in the conversion of thymine to cytosine 31.99% and 20.43%, respectively, and for the conversion of cytosine to thymine was 47.05 and 16.04%, respectively The primer of its, 5 rbcl samples identified all 8 specimens, so the rbcl primer is more suitable for suckling.