Comparison of osteopromotion ability of human tooth root powder with the demineralized freeze-dried bone allograft, a bovine xenograft and a synthetic graft. (an invitro study)

The aim of the study was to evaluate osteopromotive ability of human tooth powder and compare it to a bovine xenograft, a synthetic material and the DFDBA allograft.

This was an in vitro study. 30 teeth without caries, inflammation, infection, which have been extracted due to orthodontic reasons, have been gathered. The crowns were removed and they were treated with pulpectomy and then grinded to a powder with particles less than 500 microns. Osteoblast-like cells of MG-63 was cultured with tooth powder, Cerabone, DFDBA and Osteon II. Cell proliferation was assessed by MTT test in 24 and 72 hours. The Alizarin red test was done after 3 and 5 days. To assess the osteoblastic activity, amount of Alkaline Phosphatase was measured in 24, 48 and 72 hours. The results were analyzed by one-way ANOVO analysis.

According to the MTT test, all of the materials had a higher proliferation rate than the control group in 24 hours. In 72 hours, DFDBA with concentrations of 40 and 80 mg/ml had the lowest cell proliferation rate. DFDBA and the positive control group was able to create calcified nodules by Alizarin red test. In 48 and 72 hours, DFDBA with concentration of 40 mg/ml had the lowest alkaline phosphatase activity. In 72 hours, bovine xenograft had the highest alkaline phosphatase level and the synthetic material and tooth powder were after that.

Tooth powder was able to increase cell proliferation in comparison with the bovine xenograft, the synthetic graft and the DFDBA. However, its osteopromotive ability was less than the osteogenic materials.