Detection of bla TEM, bla OXA and bla SHV genes in Escherichia coli isolated from colibacillosis in poultry by multiplex-PCR

Antimicrobial resistance exhibited by extended-spectrum b-lactamases (ESBLs) production by E. coli is considered to be a major threat for colibacillosis in poultry. The present study deals with the detection of bla TEM, bla OXA and bla SHV genes in E. coli isolated from colibacillosis by MPCR.A total of 60 strains of E. coli were recovered from the poultry in Kerman, Iran. Cellular DNA was extracted by CinnaPure-DNA (Cell culture, Tissues, Gram negative Bacteria and CSF) and MPCR was performed for the identification of the OXA, SHV and TEM genes.CDT test results showed that 45 strains (75%) were noted for ESBL production. A total of 19 and 13 strains (31.6% and 21.6%) were positive for blaOXA and bla aada, respectively and 7 isolates (11.6%) carried both OXA and aada genes. All isolates were negative for TEM and SHV genes.The results showed, although disk diffusion is a highly practical and cost effective method for identification of ESBLs, but the novel multiplex PCR assay designed in this study may be routinely used in veterinary diagnostic laboratory for identification of extended spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae.