Evaluation of cytotoxicity effect of Synthetic Pyridopyrimidine derivatives on the cancer vs. normal cell lines

Recently, studies have been reported in vitro and in vivo, that these pyridopyrimidine compounds have a beneficial effect on chronic diseases such as cancer. this study was designed to evaluate the effects of synthetic pyridopyrimidine compounds on the growth and proliferation of normal and cancer cell lines.

Cytotoxic effect of synthetic pyridopyrimidine at different concentrations and period on the MCF-7, MAD-MB-231, MOLT-4, PC-3, and Normal Fibroblast (HFF) cell lines were cultured in 96-well plates, thin cells were treated with synthetic compounds at different concentrations 1000, 500, 50, 5 nM at 24,48 and 72 hours, to evaluate the effect using MTT assay. the resulting data were analyzed using SPSS16 software with one-way ANOVA followed by the Tukey test and P <0.05 was considered as a significant difference.

The results of this study showed that pyridopyrimidine compounds had no significant toxicity effect on the normal cell lines, Nevertheless showed strong and dose-dependent inhibition of cancer cell growth at P-value <0.05. also, results showed that pyridopyrimidines with bromide groups at a concentration of 500 to 1000 nM had a more effective effect on inhibition of cancer cell growth inhibition at 72 hours.

Results of this study indicated the pyrimidines inhibition effect on cancer cell lines, however, the use of these compounds as an attractive option in chronic diseases treatment in the world as alternative medicines or combination with conventional medicines are suggested