Investigation of Structural and biological properties of chitosan/nano-flourhydroxyapatite composite
In this study CS/nFHA composite at different compositions 90/10-50/50-40/60 w/w synthesized by sol-gel method. This nanocomposite is used as biocompatible materials for bone tissue engineering. The appropriate amounts of P/F solution was added dropwise to the Ca solution to yield a stoichiometric ratio of Ca/P1.67 and P/F6. The Phase analysis, powder morphology, the bonds configuration, functional groups, thermal behaviors and biological assesment of the sinthesized samples were studied by X-ray Diffraction XRD, Scanning Electron Microscopy SEM, Fourier transform infrared spectroscopy FTIR, Thermo-gravimetric and Differential Thermal Analysis TG-DTA and Cell culture method respectively. The XRD and SEM results showed that increasing chitosan content in the composite decreased the average FHA nanocrystallite size. The results of FTIR showed the presence of flour-hydroxyapatite phase. The result of Cell culture indicates that the addition of 10 w/w FHA has a positive effect on the cell proliferation.
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