Isolation and identification of halophilic bacteria producing asparaginase enzyme from Urmia lake

Isolation and identification of saline bacteria producing Asparaginase from Urmia Lake and effect of different salt concentrations and temperature on Asparaginase production.

The water and mud samples from Urmia Lake were cultivated in MH and SWN media for isolation of salt tolerant bacteria. Then, the Asparaginazase production rate was investigated in saline M9. The Asparaginazase production rate by isolates was investigated in diferent saline concentration (10, 20, 30, 40%) and temperatures (30, 35, 37, 45, 50˚C). Two isolates with high production rate of Asparaginase were selected, and were identified by PCR amplification and 16SrRNAs sequencing.

In present study 57 isolates were obtained which 10 isolates showed Asparaginase production. Among these, 2 isolates (A2 and A3) were selected as superior isolates. The isolate A3 was showed largest halo (54 mm) in 35˚C and 10% salt concentration. Moreover, the isolate A2 was showed largest halo (56 mm) in 35˚C and 40% salt concentration. The 16SrRNAs sequencing showed that the A2 isolate was similar 98.9% to the Halomonas elongata strain, and the A3 isolate was similar 100% to the Bacillus aryabhattai strain.

The bacteria isolated from Urmia lake are potential source of Asparaginase enzyme. The H. elongate and B. aryabhattai strains isolated from Urmia lake can be used to production of Asparaginase without side effects for cancer patients.