A study on histological changes of testis in micetreated with intratesticular gentamicin

Arrestment of spermatogenesis in the recipient is an important step in spermatogonial stem cell transplantation.Studies have shown toxic effects of gentamicin on proliferation and differentiation of bone marrow stem cells. Thepresent study was conducted to evaluate the effect of intratesticular administration of gentamicin on induction ofazospermia in mice. Twelve male mice were randomly assigned to three experimental groups: in control group,the mice received no treatment. In saline group, the mice received 0.02 ml saline intratesticularly. In gentamicingroup, the mice received 0.02 ml gentamicin (40 mg/ml) intratesticularly. Two months after treatment, the testeswere removed and their weight was recorded. Then, the specimens were fxed in 10% formalin solution, stainedand evaluated using light microscope. There was no difference among experimental groups in testicular weight andmortality (P> 0.05). Although germ cells were depleted in saline and gentamicin groups, spermatogenesis was notarrested in none of these groups based on histopathological examination. In conclusion, the results indicated thatintratesticular administration of gentamicin might not serve as an effective method for induction of azospermia inmice.