The Study of organic acids and Calcium effect on the improvement of indices of growth and oxidative induction in two cultivars of Canola (Brassica napus L.) under Aluminum stress

Aluminum toxicity causes neural, pulmonary and renal diseases in humans and reduces crops yield and growth. The factors causing the environmental toxicity of Aluminum include the increase in the ratio of rainfall to soil evaporation, soils acidification and industrial activities. Canola, the third largest oilseed in the world, has 40% to 45% oil and at least 34% protein, and due to the increase in its crop area in the world, the study of the effect of Aluminum toxicity on its growth and physiology is important. In this research, the reaction of two cultivars of rapeseed (Brassica napus), including Ocapi and Option cultivars to 80μm Sulfate Aluminum toxicity, and resistance by ligands containing 1mM of Calcium Chloride and 2mM mixture of three organic acids Citrate, Malate and Oxalate were evaluated in a hydroponic culture medium. The parameters of germination percentage, root organic acid secretion, growth parameters, anti-oxidant and enzyme indices were also measured. The results showed that Calcium ion and organic acids had a significant effect on the reduction of toxic and oxidative Aluminum effects on Canola cultivars. As a result of the addition of Calcium to a nutrient-containing nutrient solution, less organic acids are secreted, indicating a greater correlation between plasma membrane and reduced Aluminum absorption. In this study, the Ocapi variety was more responsive to the alternative than the test ligands for resistance to Aluminum stress, and this can be used to reduce the absorption of toxic metals by plants in contaminated soils and the design of new fertilizers.