Evaluation and comparison of the most important secondary metabolites of methanolic extract of different artichoke (Cynara scolymus L.) organs under drought stress and planting density

In this project, in order to evaluate and compare the most important phenolic compounds in extract of different artichoke (Cynara scolymus L.) organs under water stress and planting density, an experiment was conducted as split plot in a randomized complete block design with three replications at Sarabelle-Ilam Agricultural Research Station during 2014-2015. The treatments comprised four irrigation regime (25, 50, 75 and 100 % of the water requirement of the plant) in the main plots and four densities(10, 20, 30 and 40 thousand bushes in hectare with 0.5, 0.67, 1 and 2m interval within the row, respectively, and 0.5m between rows) in sub plots. herbal sampels were harvested in the second year, and after drying in room temperature were used for methalonic extract. Identification and amount of naringin, caffeic and chlorogenic acid was done with HPLC methode. According to the obtained results, increasing drought stress caused 78 and 11% reduction in naringin amount compared to control treatment of bud and leaf extract, respectively. The maximum amount of caffeic acid (0.74 mg/g dry matter) was observed in leaf extract and interaction effect of full irrigation and 10 thousand bushes in hectare. Drought improved caffeic and chlorogenic acid biosynthesis of bud, but in interaction effect with density, only chlorogenic acid had the highest amount in sever water stress and 10 thousand bushes in hectare .Among consumed organs extract, caffeic acid and naringin had the highest amount in leaf extract, and chlorogenic acid in bud extract