Effect of impermeable protectant addition on cryopreservation and quality indices of Acipenser persicus sperm

The aim of this study was to investigate the effect of bovine serum albumin (BSA), lecithin and trehalose on qualitative indices of freeze-thawed Acipenser persicus sperm. In this regard, sperm samples prepared from 5 male A. persicus and the samples were frozen after dilution with various extenders. After 3 months of freezing, the freeze-thawed sperm quality indices were evaluated by Image J software and mitochondrial activity by using the fluorescent staining JC-1 method. Results of sperm motility showed rotational motions in treatment BSA and linear motions in treatment “BSA + trehalose”. Analysis of motion parameters in freeze-thawed sperm showed that the treatment “BSA + trehalose” had the highest arc velocity (1.74μM/s), highest linear velocity was in BSA and trehalose treatments (0.30µM/s) and the highest linear movement was seen in trehalose and trehalose + lecithin treatments (23.87 and 25.48%, respectively). Results of the effect of preservatives on sperm motility showed the highest percentage in treatment “BSA + trehalose” (3.49%) and the highest mitochondrial activity in trehalose treatment (10.58%). Therefore, the use of BSA with trehalose can have a potential impact on reducing cell damage during cryopreservation, the protection and development of this valuable species of Caspian species.