Dispersive micro solid phase extraction based on surface magnetic molecular imprinted polymer and deep eutectic solvent with optimization by central composite design for determination of dipyridamole in pharmaceutical and biological samples

In this work, a simple and inexpensive dispersive micro solid-phase extraction method using magnetic molecular imprinted polymer nanocomposite and deep eutectic solvent and optimization by central compound design method was developed for selective determination of dipyridamole in pharmaceutical and biological samples. The synthesized nanocomposite was used to enhance the extraction efficiency of the adsorption and desorption step, also a deep eutectic solvent was used for the desorption of the analyte and the detection was performed by high performance liquid chromatography with an ultraviolet detector. Simultaneous optimization of effective parameters include the adsorbent dosage (w), desorption sovent volume (v), and desorption time (t) was performed by the central compound design method. After optimization, the maximum adsorption capacity of the analyte on the magnetic molecular imprinted polymer adsorbent for dipyridamole 2.82 mg g-1 and the obtained linear range for the method was 0.008-1200 μg L-1. Limit of detection and quantitation were also obtained 0.003 µg L-1 and 0.009 µg L-1, respectively. The relative standard deviation of the method by inter-day test for concentration levels of 5, 50 and 150 µg L-1 were 3.26%, 2.09% and 3.41%, respectively, and by intra-day test for the same concentration levels were less than 4%. . Finally, this very method was successfully used for preconcentation and determination of dipyridamole in plasma, serum and tablet samples with a standard deviation of less than 4%. Keywords: Dipyridamole; Micro solid phase extraction; Magnetic nanoparticles ; Molecular imprinted polymer; Deep Eutectic Solvent; Central composite design