Improvment of Skin injuries and Performance of Animal, Cellular and Clinical Tests by Starch/ HA / Royal Jelly / PU Scaffold Production

Existing dressings do not have the ability to protect against damage and contamination by microorganisms ideally. The aim of this study is to obtain a scaffold of polyurethane – starch, royal jelly and hyaluronic acid by electrospinning method and a scaffold with suitable biological properties for wound healing is made.

To make a nanofiber scaffold, first 10% by weight / weight of polyurethane is dissolved at room temperature for 2 hours. Royal jelly was added to the polyurethane solution in 3, 6 and 8% ratios, starch and hyaluronic acid (1% by weight / weight) and electrospinning was performed. The scaffold prepared were characterized SEM, MTT, cell culture and in-vivo of the scaffolds.

With the addition of royal jelly, the viscosity of the solution is increased and the diameter of the electrospun fibers is increased. In the MTT test, L929 fibroblast cells showed much better proliferation on polyurethane – starch - hyaluronic acid - royal jelly than other scaffold. Animal studies show that the percentage of recovery in the group treated with polyurethane-starch, containing hyaluronic acid and royal jelly scaffold from the seventh to the fourteenth day after the injury is higher than the percentage of wound healing in other groups and significant control group.

Polyurethane-Starch electrospun scaffold with a content of hyaluronic acid (1% w/w) and Royal Jelly (6%) is prepared which is biologically suitable for use in skin tissue to heal wounds. Royal Jelly improves cell proliferation and adding it to scaffold also increases cellular adhesion to scaffolding. The findings of this study suggest that the use of this electrified scaffold can be effective in wound healing.