Nucleotide Sequence of Gene Encoding Capsid Protein VPI of Foot-and-Mouth Disease Virus / Type O1 Iran
VI' 1 protein of foot-and-mouth disease ViruS (FMDV) contains the immonogenic hypervariable region of the virus. The antigenic variation in FMDV is particularly related to the difference between nucleotidc and amino acid sequences of capsid protein. On the basis of this phenomenon. type diagnosis of FMDV can be done by the polymerase chain reaction (PCR). In order to specifically identify the 0, FMDV serotype of Iran the complete coding sequence of its VPI prote in was amplified by RT-PCR, and nuclcotidc and amino acid sequences of the PCR product were dctermincd. The nuclcotide and deduced amino acid sequence exhibited 84% and 88% homology with the VP 1 region of serotype O,K. respectively.
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