Preparation of Semi-purified Laminated Layer of Hydatid Cyst for Diagnosis of Infection

A semi-purified laminated layer (LL) fraction of cyst wall of Echinococcus Kranulo.\u.\ was prepared by affinity chromatography. Aiso its antigenic reactivity evaluated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SOS-PAGE). SOS-PAGE analysis of the LL fractions showed that the bands in the 50-55kOa region and the 25-31 kOa region were greatly reduced in semi-purified fraction in comparison with unpurified sample, which presumably reflected to removal of heavy and light immunoglobulin chains. An immunoblot data also confirms considerable reduction in the presence of IgG chains within the semipurified fraction. The current study indicated that the bands at 66, 55 and 27kOa were significant molecules for diagnosis of human cystic hydatidosis patients after purification of the crude sample.