Effect of Bacteriocin Produced by Lactobacillus casei with Probiotic Potential Isolated from Milk on the Ability of Biofilm Production by Streptococcus salivarius Obtained from Dental Plaque

Dental plaque is a biofilm that is formed on the surface of the tooth. Carious lesions are caused by inappropriate ecological changes in microbial flora of the plaque biofilm. In this study, the effect of Lactobacillus casei bacteriocin, isolated from dairy products, was investigated on Streptococcus salivarius biofilm formation.

In this experimental study, MRS Broth and MRS Agar medium were used to isolate three strains of Lactobacillus casei probiotic from milk samples under microaerophilic conditions. The initial identification of Lactobacillus casei was performed by sugar fermentation and other biochemical tests. Then, 16SrRNA encoding gene was confirmed by PCR and sequenced. At last, Bacteriocin was isolated by ammonium sulfate sedimentation and its molecular weight was measured by SDS-PAGE and the antimicrobial activities were investigated. The Streptococcus salivarius were isolated from dental plaque and phenotypically and biotypically identified. Molecular confirmation of the isolates was performed using gtfK specific gene by PCR. The ability to form Streptococcus salivarius biofilm and the effect of bacteriocin on biofilm formation were measured by microtiter plate.

After partial purification of Lactobacillus casei probiotic from dairy products, two LC3 and LC1 protein samples formed bands (35-40 kDa and about 75 kDa, respectively). The supernatant fluid from the cultivation of Lactobacillus casei and bacteriocins showed antibacterial effects against Streptococcus salivarius isolates from dental plaque and the formation of biofilm by this bacterium.

According to this study, bacteriocin and supernatant fluid from Lactobacillus casei cultures could inhibit the growth of Streptococcus salivarius and also reduced biofilm formation, so, it could be used in oral care products.