Histometric Study the Effects of Thiamin on the Structure of the Small Intestine in Induced Alloxan Diabetic Rats

Diabetes is characterized by high blood glucose levels and defects in insulin production and function. Contamination of food and the environment with lead can worsen the condition of diabetics. This study investigated the effect of thiamine on the small intestinal histomorphology of alloxan-induced diabetic rats.

In this interventional study, 63 Wistar rats were randomly divided into 9 groups. Three groups of animals were considered as control group (A), thiamine+lead 200 (H) and thiamine+lead 1000 (I). Lead in ppm and acetate form was added to drinking water. The other groups became diabetic via alloxan at a dose of 200 mg/kg. Group B: diabetic mice; Group C: Diabetic mice receiving thiamine; Groups D and E of diabetic mice receiving lead 200 and 1000 were named. Mice in groups F and G received 200 and 1000 ppm of thiamine and lead. Thiamine was used daily and peritoneal with 70 mg/kg dose. On the 29th day, histotechnique was performed on small intestine samples and their histomorphometry was examined.

Histomophometry of intestinal structure in mice receiving untreated lead in diabetic group showed a significant increase compared to healthy mice. However, these parameters did not show a significant difference in lead-receiving diabetic rats treated with thiamine compared to the control group. The thiamin improved insulin secretion and reduced turbulence in villi´s of small intestine and improve size of muscular layer in digestive lumen.

Probably the antioxidant compound in the thiamin can reduce some complications of lead acetate in diabetic patients.