The Effect of Eight Weeks of Endurance Training with Saffron on miR133bFC, miR29aFC in the Hippocampus Tissue and Depression in Rats with Alzheimer’s Disease

 Recent studies indicate that deregulation of microRNAs (miRNAs) expression is associated with neurological and cognitive disorders, but physical activity and medicinal plants have favorable effects on physiological and psychological factors in these patients.

 Therefore, we aimed to investigate the effect of endurance training (ET) with saffron (S) on miR133bFC, miR29aFC in the hippocampus tissue and depression of rats with Alzheimer’s disease (AD).

 Forty AD rats with the mean age of eight weeks and mean weight of 250 ± 30.65 g were randomly divided into five groups of eight rats including: (1) control (C), (2) ET, (3) ET + S, (4) S, and (5) sham (normal saline) (Sh). During eight weeks, groups 2 and 3 ran on a treadmill for three sessions per week, each session lasting for 15 - 30 minutes, at a speed of 20 - 15 m/min, and groups 3 and 4 received 25 mg/kg daily aqueous extract of S peritoneally. Depression was evaluated by the forced swim test.

 The levels of miR29aFC were higher in the ET + S group than in the C (P = 0.002), Sh (P = 0.003), ET (P = 0.003), and S (P = 0.001) groups. The levels of miR133bFC in the S (P = 0.02) and ET (P = 0.005) groups were lower than the C group. The mobility time in the ET (P = 0.001), S (P = 0.001), and ET + S (P = 0.001) groups was higher than the C group; in the ET + S group, the mobility time was higher than in the ET (P = 0.001) and S (P = 0.001) groups, and in the S group the mobility time was higher than in the ET group (P = 0.001).

 It seems that ET and S administration alone do not have favorable effects on miR29aFC and miR133bFC expression levels, but both can decrease depression; however, the simultaneous administration S and ET has interactive effects on improving miR29aFC expression and reducing depression.