Studying Some Blood Parameters of Otolithes ruber (Schneider, 1801) in Cold and Warm Seasons as an Indicator of Pollution in Musa Creek

The present study attempts to investigate some blood parameters of Otolithes ruber during different seasons in terms of both temperature and pollution. For so doing it uses 10 specimens, for each station and season, collected from 5 polluted stations, including Petrochemical, Ghanam, Zangi, Douragh, Patil, and Sajafi as the control group, away from pollution in Musa Creek. The fish are anesthetized with 1ml of clove extract per liter. Their blood samples are taken immediately from the caudal vein, using a heparinized syringe. Afterwards, the serum is separated in a centrifuge with a speed of 6000 rpm for 2 minutes. The desired factors are measured by the Mindray BS200 auto-analyzer and the total protein level, by Bradford's usual laboratory methods. Results show that AST, ALT, ALP, Glucose, and Triglycerides have increased in more polluted stations (P≤0.05). In sheer contrast, total protein and Albumin have decreased as pollution grows (P≥0.05). According to this study, environmental water pollution of the fish has a large impact on the concentration of measured blood parameters, whereas the influence of seasonal changes on most of them is low.