Identification of extended-spectrum β-lactamase genes in Escherichia coli isolated from patients with urinary tract infection

Recentlly, resistance to antibiotics has increased and antibiotic-resistant strains producing extended-spectrum beta-lactamases (ESBLs) have emerged among Enterobacteriaceae, mainly in Escherichia coli (E. coli). In this study we aimed to determine phenotypic and genotypic ESBL production in isolated E. coli from women with urinary tract infection (UTI).

In total, 92 E. coli isolates were collected from patients with UTI. The antimicrobial susceptibility of all E. coli isolates were investigated. Morover, Mast D68C test and polymerase chain reaction (PCR) were used for phenotypic and genotypic investigation of ESBLs in the studied isolates.

Totally, 92 isolates of E. coli were investigated, among which 51 (55.4%) isolates were resistant to cefotaxime/ceftazidime. Theise resistante isolates were included in the study. Among the resistant isolates, 40 (78.4%) cases were ESBL producers. Moreover, all the 40 isolates were observed with both CTX-M-15 and CTX-M-14 resistance genes.

In general, high increasing prevalence of ESBL producer E. coil isolates of E. coil is a serious problem in the investigated region. Therefore, development of a rapid and simple method is essential to for the identification of various ESBL producer isolates.