Synthesis of Silver Nanoparticles with Sodium Brohydrate by Chemical Revival Method to Produce Biodegradable Antibacterial Silver Nanocomposite by Solution Blending Production of biodegradable antibacterial silver nanocomposites

This study aimed to investigate the antibacterial effect of silver nanoparticles on Gram-negative and Gram-positive bacteria and producing silver nanocomposites with suitable and biodegradable antibacterial properties by Solution Blending method.

To investigate the antimicrobial properties of silver nanoparticles, silver nanoparticles were first synthesized by NaBH4 reduciton and after performing UV-VIS, DLS, XRD and TEM and FT-IR  tests, Staphylococcus aureus as a Gram-positive, Escherichia coli as  a Gram-negative bacteri was used, investigated by MIC, MBC and Inhibition Zone. In the next step, the nanocomposite was prepared and prepared by Solution Blending method and its antibacterial activity was evaluated by Inhibition Zone method.

The results showed that silver nanoparticles had inhibitory effect on Staphylococcus aureus, Escherichia coli and Candida albicans at concentrations of 50, 20 and 355 μg / ml respectively and also at concentrations of MIC. 60, 40 and 370, respectively, have the lethal effect on the aforementioned microorganisms (MFC, MBC). A diameter of 3-5 mm was observed around the silver nanoparticles in the cultures of the mentioned microorganisms and in the test of nanocomposite Inhibiion zone, the halo was created based on expected values.the results of the experiments were calculated with 3 replications and the amount of PValue was significant (P<0/0001)by ANOVA method.we used also 3 antibiotic as control for Inhibition zone test.

Silver nanoparticles showed good inhibitory and lethal effects on Gram-positive and Gram-negative bacteria and Also, the nanocomposites produced by the mentioned method have high antibacterial and viscolastic properties.