Phylogenetic status of the endophyte Bacillus sp. Bp91 isolated from potato and its antibacterial activity against Ralstonia solanacearum

Bacterial wilt disease caused by Ralstonia solanacearum is one of the most destructive diseases in the world, including Kurdistan province of Iran. Using the endophytic bacteria is one of the most effective and applicable methods to control of the disease. In this study, Bacillus sp. Bp91 which was isolated from potato fields in Kurdistan province was used as a superior antagonist against R. solanacearum. The results of the supplementary tests showed that the production of protease in the mentioned strain is positive. This was negative and relatively weak for hydrogen cyanide and siderophore production, respectively. The phylogenetic tree derived from 16S rRNA gene sequence showed the stain belongs to the genus Bacillus sharing the highest level of sequence homology with to B. pumilus. Metabolic studies by GC-mass were also indicated that the two metabolites Eicosane and Dibutyl phthalate are the most prominent antimicrobial metabolites produced by the bacterium.