Assessment of Pili Gene in Susceptible and Drug-Resistant Mycobacterium Tuberculosis

Mycobacterium tuberculosis (M. tuberculosis) is responsible for a large number of human deaths because of its ability to remain in the host for many years without causing disease. Recently, new areas with high prevalence of MDR-resistant TB have been identified in populous countries. Pili in M. tuberculosis mediates the interaction between the pathogen and the host-specific target cells and is seen as a factor in the pathogenesis of the bacterium. In this study, we examined producing gene of (Mtp) among susceptible and resistant strains isolated from clinical specimens to better understand the biology of M. tuberculosis.
We have used some PCR-based methods, including: ISR10 PCR, Multiplex-PCR as fast molecular methods. 100 patient samples were identified by IS6110 PCR method, then isolation of multidrug resistance and sensitive and resistant strains was performed.
Dedicated band electrophoresis showed 263 bp for the Mtp gene. The results of the present study show that this gene is present in all considered strains.
The pili gene is protected in Mycobacterium and is present in all susceptible and drug-resistant strains.