Investigation of Long Non-coding RNA CRNDE Expression in Iranian Patients with Colorectal cancer

This study aimed to compare the expression levels of LncRNA CRNDE in malignant colorectal tumorsand adjacent normal tissues of patients by Real Time-PCR.

20 pairs of colorectal tumors and adjacent normal tissue specimens were prepared from the tumor bank of Imam Khomeini Hospital in Tehran University of Medical Sciences. After total RNA extraction from cancerous and normal tissues, cDNA was synthesized, and the expression levels of LncRNA CRNDE were examined using the Real Time-PCR method. A one-way analysis of variance (ANOVA) and Tukey's HSD test used for statistical analysis of results.

The results showed a two-fold increase in LncRNA CRNDE expression in all 20 colorectal cancer samples compared with the normal cells.

According to the significant increase of the LncRNA CRNDE expression in cancer cells relative to normal cells and its presence in the bloodstream, this LncRNA maybe used as a non-invasive diagnostic biomarker for early detection of colorectal cancer.