Effects of Hypertonic saline and Gelatin solutions on plasma levels of lactate and electrolytes in an experimental endotoxemic sheep model

To evaluate the effects of hypertonic saline and gelatin solutions on plasma lactate concentration and serum electrolytes following experimental endotoxemia, twenty sheep were randomly divided into 4 groups: the negative control, positive control, hypertonic saline and gelatin. Endotoxaemia was induced in the gelatin, hypertonic and positive control groups by intravenous administration of lipopolysaccharide (LPS) from E. coli serotype O55:B5 at 0.1 µg/kg. Serum lactate and electrolytes were measured 30 min before and 30 min after LPS injection, as well as on mins 90 after LPS injection, 30, 60, 90, 120, 150, 180 and 240 min after injection of the solutions. The results showed that plasma lactate significantly increased immediately after endotoxemia induction but a significant decrease was observed in the hypertonic saline group on 90 minutes after injection and in the gelatin group 60 minutes after injection. Serum potassium concentration after endotoxemia induction was significantly decreased compared to the negative control group and also decreased significantly after 90 min. Serum sodium and calcium concentrations decreased after induction of endotoxemia and increased after treatment with solutions. Serum phosphorus concentration increased with induction of endotoxemia and decreased after injection of solutions. In this study, it was found that hypertonic saline and gelatin solutions make blood lactate and electrolytes close to normal after induce of experimental endotoxemia in sheep model.